Composite

Part:BBa_K329057

Designed by: Xavier Duportet   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-25)

[KanR + LacZ] in between MUT_MUT[HK022] Tn916 Arms

This part is composed of a Kanamycine resistance and LacZ cassette between two mutated arms (left and right) of the transposon Tn916 containing each of us one half of the attR HK 022 recombination site. When the both enzyme of the transposon,Int and Xis tn916 are expressed (see BBa_K329035 and BBa_K329018)., the both arms link together forming a circular DNA sequence excised from the hosting DNA (plasmid or chromosome). The excision permit to create a functionnal attR HK 022 recombination site. This mutant provide a high efficiency excision characterized (see experience part).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 382
    Illegal NheI site found at 405
    Illegal SpeI site found at 363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 363
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None